Format
96-WellPlate
SpeciesReactivity
Homosapiens(Human)
SampleType
Tissuehomogenates,Celllysatesandother
BIOLOGicalfluids
DetectionRange
0.312-20ng/mL
Sensitivity
0.129ng/mL
EstimatedTurnaroundTime
7-11businessdays
TargetName
CalciumChannel,VoltageDependent,N-Type,Alpha1BSubunit
TargetSynonyms
CACNN,CACNL1A5,BIII,BraincalciumchannelIII,Calciumchannel,Ltype,alpha-1polypeptideisoform5,Voltage-gatedcalciumchannelsubunitalphaCav2.2
Precision
Intra-assayPrecision(Precisionwithinanassay):3sampleswithlow,middleandhighlevelCalciumChannel,VoltageDependent,N-Type,Alpha1BSubunitweretested20timesononeplate,respectively.Inter-assayPrecision(Precisionbetweenassays):3sampleswithlow,middleandhighlevelCalciumChannel,VoltageDependent,N-Type,Alpha1BSubunitweretestedon3differentplates,8replicatesineachplate.CV(%)=SD/meanX100;Intra-Assay:CV<10%;inter-assay:>10%;inter-assay:><>
DetectionMethod
Colorimetric
AssayTime
3hours
Application
AssayType
Double-antibodySandwich
ShippingCondition
Icepacks
Storage
Shortterm:4°C;Longterm:seemanual.
PrecautionofUse
TheStopSolutionisacidic.Donotallowtocontactskinoreyes.
ExpiryDate
8months
UniProtID
Q00975
Specificity
ThisassayhashighsensitivityandexcellentspecificityfordetectionofCalciumChannel,VoltageDependent,N-Type,Alpha1BSubunit
CrossActivity
Nosignificantcross-reactivityorinterferencebetweenCalciumChannel,VoltageDependent,N-Type,Alpha1BSubunitandanalogueswasobserved.
AssayProcedure
1.Prepareallreagents,samplesandstandards;2.Add100µLstandardorsampletoeachwell.Incubate2hoursat37°C;3.Aspirateandadd100µLpreparedDetectionReagentA.Incubate1hourat37°C;4.Aspirateandwash3times;5.Add100µLpreparedDetectionReagentB.Incubate30minutesat37°C;6.Aspirateandwash5times;7.Add90µLSubstrateSolution.Incubate10-20minutesat37°C;8.Add50µLStopSolution.Readat450nmimmediately.
Thestabilityofkitisdeterminedbythelossrateofactivity.Thelossrateofthiskitislessthan5%withintheexpirationdateunderappropriatestoragecondition.Tominimizeextrainfluenceontheperformance,operationproceduresandlabconditions,especiallyroomtemperature,airhumidity,incubatortemperatureshouldbestrictlycontrolled.Itisalsostronglysuggestedthatthewholeassayisperformedbythesameoperatorfromthebeginningtotheend.
Restrictions
ForResearchUseOnly.Notforuseinvitrodiagnosticprocedures,druguse,orforadmi
NISTrationtohumansoranimals.
QualitySystems
ThekitismanufacturedatISO9001andISO13485certifiedfacilities.
QualityAssurance
BiomatikELISAkitsarenottypicallypre-assembledasfinishedproductsduetolimitedshelflife.Beforeshipping,eachkitisassembledandtestedtoensurethatitmeetsspecificationsbeforeshipping.MinorchangesmayoccurtotheDetectionRange,Sensitivity,andPrecision.AllkitsaretestedtoconfirmthattheyfallwithintheirdefinedInter-andIntra-assaycoefficientofvariation.PleasealwaysrefertothehardcopymanualincludedinThekitbeforeexperiment.
ResearchArea
Signaltransduction