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主营:ELISA试剂盒,蛋白质,抗体,生化试剂,基因合成,多肽合成,蛋白表达,抗体生产
℡ 4000-520-616
℡ 4000-520-616
Biomatik/Mouse Alpha-Fodrin (SPTAN1) ELISA kit, Cat#EKU02297/96 Tests/EKU02297-96 Tests
产品编号:EKU02297-96Tests
市  场 价:¥13600.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$680.00
品      牌: Biomatik
公      司:Biomatik
公司分类:
Biomatik/Mouse Alpha-Fodrin (SPTAN1) ELISA kit, Cat#EKU02297/96 Tests/EKU02297-96 Tests
商品介绍

Format

96-Well Plate

Species Reactivity

Mus musculus (Mouse)

Sample Type

Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids.

Detection Range

1.56-100 ng/mL

Sensitivity

0.68 ng/mL

Estimated Turnaround Time

7-11 business days

Target Name

Alpha-Fodrin

Target Synonyms

SPTAN1, NEAS, (ALPHA)II-SPECTRIN, Spectrin Alpha Non-Erythrocytic 1, Spectrin Alpha Chain, Brain, Spectrin, non-erythroid alpha subunit

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Fodrin were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Fodrin were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV<10%;inter-assay:><>

Detection Method

Colorimetric

Assay Time

3 hours

Application

ELISA

Assay Type

Double-antibody Sandwich

Shipping Condition

Ice packs

Storage

Short term: 4°C; Long term: see manual.

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Expiry Date

8 months

UniProt ID

P16546

Specificity

This assay has high sensitivity and excellent specificity for detection of Alpha-Fodrin

Cross Activity

No significant cross-reactivity or interference between Alpha-Fodrin and analogues was observed.

Assay Procedure

1.Prepare all reagents, samples and standards; 2.Add 100 µL standard or sample to each well. Incubate 2 hours at 37°C; 3.Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4.Aspirate and wash 3 times; 5.Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6.Aspirate and wash 5 times; 7.Add 90 µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8.Add 50 µL Stop Solution. Read at 450 nm immediately.

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Restrictions

For Research Use Only. Not for use in vitro diagnostic procedures, drug use, or for administration to humans or animals.

Quality Systems

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Quality Assurance

Biomatik ELISA kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in The kitbefore experiment.

Research Area

Signal transduction, Apoptosis
品牌介绍

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产品涵盖生化试剂、重组蛋白、ELISA试剂盒、基因定制和多肽合成及蛋白及抗体的定制生产等。始终把科研人员的科研需要摆在第一位,为用户独一无二的研究项目提供完美的产品和服务。Biomatik为科研人员提供工业领先的创新产品和高效的客户服务。公司在为用户提供高质量的产品,满足用户科研需要的同时,也为用户节省研究经费,努力创造双赢的局面~~~~~~

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